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ISSN:0122-8706 E-ISSN: 2500-5308 DOI: 10.21930
10.21930/rcta.vol25_num3_art:3662
Enviado: 2023-12-30
Publicado: 2024-12-26

Efecto de la desinfección, el desarrollo foliar y las fitohormonas en la inducción de callos de Simarouba amara Aubl.

Universidad Nacional de Ucayali
Universidad Nacional Intercultural de la Amazonia
Universidad Nacional Intercultural de la Amazonía
Universidad Nacional de Ucayali
Universidade Federal do ABC
Universidade Federal do ABC
Universidad Nacional Intercultural de la Amazonía
Universidad Nacional de Ucayali

Cómo citar

Efecto de la desinfección, el desarrollo foliar y las fitohormonas en la inducción de callos de Simarouba amara Aubl. (2024). Ciencia Y Tecnología Agropecuaria, 25(3). https://doi.org/10.21930/rcta.vol25_num3_art:3662

Resumen

Disinfection and callus induction in forest species is essential to improve genetic production. Although multiple methods exist, their application in forest plants requires further research. Therefore, this study focused on the effect of disinfection, leaf development and phytohormones on callus induction in Simarouba amara. The study was structured in two experiments. In the first experiment, the efficacy of leaflet disinfection was evaluated using a completely randomized design with three sodium hypochlorite treatments (0 %, 0.25 % and 0.5 %). The second experiment, focused on callus induction, was developed under a 3 x 6 factorial design, which included three stages of leaf development (young, intermediate and mature) and six combinations of phytohormones (BAP, TDZ, 2,4-D, BAP+TDZ, BAP+2,4-D, TDZ+2,4-D, all at 1 mg/L). The main results showed that 0.25 % sodium hypochlorite achieved 88.9 % asepsis, with a lower percentage of oxidation (17 %). It was observed that, combinations of BAP+2,4-D and TDZ+2,4-D promoted higher callus formation, increased callus area and fresh and dry matter production, especially in segments of intermediate leaf development stage. This study highlights the importance of adjusting disinfectant and phytohormones according to leaf development stage for successful callus induction in Simarouba amara.

2347 1075
  • cultivo in vitro
  • hoja
  • desinfección
  • callogenésis
  • auxina
  • citoquinina
  • Simarouba
  1. Aguilera-Arango, G. A., Puentes-Díaz, C. L., & Rodríguez-Henao, E. (2021). Disinfection methods for the in vitro establishment of two varieties of cassava for agroindustrial use. Revista de Investigación e Innovación Agropecuaria y de Recursos Naturales, 8(3), 21-30.
  2. https://doi.org/10.53287/kdux7546xv19l
  3. Arbeláez, L. M., Montoya, J. L., & Saavedra, S. A. R. (2016). Assessment protocols for the establishment and disinfection in vitro meristem of Banana Musa spp. Vitae, 23(1), 391-395.
  4. Argentel, L. M., Fonseca, I. R., Garatuza, J. P., Yépez, E. G., & González, J. A. (2017). Effect of salinity on calli of wheat varieties during in vitro culture, Revista Mexicana de Ciencias Agrícolas, 8(3), 477-488. https://doi.org/10.29312/remexca.v8i3.25
  5. Assareh, M. H., & Sardabi, H. (2005). Macropropagation and micropropagation of Ziziphus spina-christi. Pesquisa Agropecuária Brasileira, 40(5), 459-465.
  6. https://doi.org/10.1590/s0100-204x2005000500006
  7. Aublet, F. (1775). Histoire des plantes de la Guiane Françoise. Londres e Paris: Pierre-François Didot. Botanicus, 2, 860-861. http://www.botanicus.org/page/361614
  8. Azofeifa-Delgado, Á. (2009). Problemas de oxidación y oscurecimiento de explantes cultivados in vitro. Agronomía Mesoamericana, 20(1), 153-175.
  9. https://doi.org/10.15517/am.v20i1.4990
  10. Bello-Bello, J. J., Martínez-Estrada, E., Caamal-Velázquez, J. H., & Morales-Ramos, V. (2016). Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews). African Journal of Biotechnology, 15(8), 272-277.
  11. https://doi./10.5897/AJB2015.14662
  12. Boyette, M. D., Ritchie, D. F., Carballo, S. J., Blankenship, S. M., & Sanders, D. C. (1993). Chlorination and postharvest disease control. HortTechnology, 3(4), 395-400.
  13. https://doi.org/10.21273/HORTTECH.3.4.395
  14. Bramhanapalli, M., Thogatabalija, L., & Gudipalli, P. (2017). Efficient in vitro plant regeneration from seedling-derived explants and genetic stability analysis of regenerated plants of Simarouba glauca DC. by RAPD and ISSR markers. In Vitro Cellular & Developmental Biology - Plant, 53(1), 50-63.
  15. https://doi.org/10.1007/s11627-016-9795-0
  16. Carbonell, X. R., Verdes, P. E., & Leporati, J. L. (2022). Preliminary evaluation of in vitro callogenesis and organogenesis Leptochloa crinita (Lag.) P. M. Peterson & N. Snow. Revista Colombiana de Biotecnología, 24(1), 19-26.
  17. https://doi.org/10.15446/rev.colomb.biote.v24n1.99374
  18. Chen, B., Li, J., Zhang, J., Fan, H., Wu, L., & Li, Q. (2016). Improvement of the tissue culture technique for Melaleuca alternifolia. Journal of Forestry Research, 27(6), 1265-1269.
  19. https://doi.org/10.1007/s11676-016-0301-7
  20. Chung, H. H., Chen, J. T., & Chang, W. C. (2007). Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium. Biologia Plantarum, 51(2), 346-350.
  21. https://doi.org/10.1007/s10535-007-0069-x
  22. Corredor, E. Y., Imakawa, A. M., Da Silva, D., & Barbosa, P. T. (2022). In vitro callus induction from different explants of Senna alata (L.) Robx. (FABACEAE). Advances In Forestry Science Cuiabá, 9(1), 1653-1660. https://doi.org/10.34062/afs.v9i1.12928
  23. Cruz, C. A. F., Paiva, H. N., & Guerrero, C. R. A. (2006). Efeito da adubação nitrogenada na produção de mudas de sete-cacas (Samanea inopinata (Harms) Ducke). Revista Árvore, 30(4), 537-546. https://doi.org/10.1590/S0100-67622006000400006
  24. Da Silva, D., Imakawa, A. M., Souza, F. M., & Barbosa, P. T. (2018). Indução de calos friáveis em explantes foliares e segmentos nodais de pau-ferro (Caesalpinia ferrea). Revista de Ciências Agrárias, 41(4), 1044-1050. https://doi.org/10.19084/RCA17311
  25. Devecchi, M. F., & Pirani, J. R. (2016). Flora of the cangas of the Serra dos Carajás, Pará, Brazil: Simaroubaceae. Rodriguésia, 67(5), 1471-1476.
  26. https://doi.org/10.1590/2175-7860201667551
  27. Dos Santos, M. R. A., Ferreira, M. D. G. R., Correia, A. D. O., & Da Rocha, J. F. (2010). In Vitro establishment and callogenesis in shoot tips of Peach Palm. Revista Caatinga, 23(1), 40-44. http://www.redalyc.org/articulo.oa?id=237117582007
  28. Fehér, A. (2019). Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean in the Era of Molecular Plant Biology? Frontiers in Plant Science, 10, 536. https://doi.org/10.3389/fpls.2019.00536
  29. Gammoudi, N., Nagaz, K., & Ferchichi, A. (2022). Establishment of optimized in vitro disinfection protocol of Pistacia vera L. explants mediated a computational approach: multilayer perceptron–multi−objective genetic algorithm. BMC Plant Biology, 22, 324.
  30. https://doi.org/10.1186/s12870-022-03674-x
  31. Gavilan, N. H., Furlan, F. C., Zorz, A. Z., De Oliveira L. S., Campos, W. F., & Brondani, G. E. (2018). Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium. Ciência Rural, 48(9), e20170581.
  32. https://doi.org/10.1590/0103-8478cr20170581
  33. GBIF. (2023). GBIF Backbone Taxonomy. Checklist dataset Simarouba amara Aubl. https://doi.org/10.15468/39omei
  34. Gomes-Copeland, K. K. P., Lédo, A. S., Juceni, P. D., Aparecida, G. A., & Fabrício, T. C. A. (2017). In vitro callogenesis of Poincianella pyramidalis (catingueira). Revista Brasileira de Farmacognosia, 27(4), 525-528. https://doi.org/10.1016/j.bjp.2016.12.005
  35. Gonzales-Alvarado, A. C., Mori-Vasquez, J. A., Tuisima Coral, L. L., & Revilla-Chávez, J. M. (2022). Influencia de la desinfección, medios de cultivo y fitohormonas en el desarrollo morfogénico in vitro de germoplasma de Guazuma crinita Mart. Folia Amazónica, 31(1), 57-70. https://doi.org/10.24841/fa.v31i1.572
  36. Gratão, P. L., Polle, A., Lea, P. J., & Azevedo, R. A. (2005). Making the life of heavy metal-stressed plants a little easier. Functional Plant Biology, 32(6), 481-494.
  37. https://doi.org/10.1071/FP05016
  38. Gu, M., Li, Y., Jiang, H., Zhang, S., Que, Q., Chen, X., & Zhou, W. (2022). Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) IM. Johnst, a Multipurpose Woody Plant. Plants, 11(15), 1937.
  39. https://doi.org/10.3390/plants11151937
  40. Gupta, S. D., & Jatothu, B. (2013). Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and morphogenesis. Plant Biotechnology Repports, 7(3), 211-220. https://doi.org/10.1007/s11816-013-0277-0
  41. Hadke, S. P., Deshmukh, A. G., Dudhare, M. S., & Vaidya, E. R. (2008). Callus induction in Simarouba glauca D.C. Asian Journal of Biological Sciences, 3(1) 1-4.
  42. Hardesty, B. D., Dick, C. W., Kremer, A., Hubbell, S., & Bermingham, E. (2005). Spatial genetic structure of Simarouba amara Aubl. (Simaroubaceae), a dioecious, animal-dispersed Neotropical tree, on Barro Colorado Island, Panama. Heredity, 95(4), 290-297.
  43. https://doi.org/10.1038/sj.hdy.6800714
  44. Hasnain, A., Naqvi, S. A. H., Ayesha, S. I., Khalid, F., Ellahi, M., Iqbal, S., Hassan, M. Z., Abbas, A., Adamski, R., Markowska, D., Baazeem, A., Mustafa, G., Moustafa, M., Hasan, M. E., & Abdelhamid. M. M. A. (2022). Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.1009395
  45. Hernández-Amasifuen, A. D., Cortez-Lázaro, A. A., Argüelles-Curaca, A., & Díaz-Pillasca, H. B. (2021). In vitro callogenesis of peach (Prunus persica L.) var. Huayco rojo from leaf explants. Ciencia y Tecnologia Agropecuaria, 23(1), e2032.
  46. https://doi.org/10.21930/rcta.vol23_num1_art:2032
  47. Hesami, M., Naderi, R., & Yoosefzadeh-Najafabadi, M. (2018). Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa. BioTechnologia, 99(1), 49-57.
  48. https://doi.org/10.5114/bta.2018.73561
  49. Hesami, M., Naderi, R., & Tohidfar, M. (2019). Modeling and Optimizing in vitro Sterilization of Chrysanthemum via Multilayer Perceptron-Non-dominated Sorting Genetic Algorithm-II (MLP-NSGAII). Frontiers in Plant Science, 10, 282.
  50. https://doi.org/10.3389/fpls.2019.00282
  51. Hill, A., De león, D., & Dervieux, C. (2022). Quillt: 'pkgdown' template for the r markdown ecosystem. https://github.com/rstudio/quillt,
  52. https://pkgs.rstudio.com/quillt.2022
  53. Hoffmann, L. T., Bittencourt, R., Grott, I. T., & Sperlich, C. L. (2022). In vitro germination of Raulinoa echinata R. S. Cowan (Rutaceae): seeds and zygotic embryos Ciência Florestal, Santa Maria, 32(3), 1187-1204.
  54. https://doi.org/10.5902/1980509837874
  55. Honda, H., Lui, C., & Kobayashi, T. (2001). Large-Scale Plant Micropropagation. Plant Cells, 72, 157-182. https://doi.org/10.1007/3-540-45302-4_6
  56. Iiyama, C. M., & Cardoso, J. C. (2021). Micropropagation of Melaleuca alternifolia by shoot proliferation from apical segments. Trees, 35(5), 1497-1509.
  57. https://doi.org/10.1007/s00468-021-02131-w
  58. IUCN. 2023. The IUCN Red List of Threatened Species. Version 2021-3. https://www.iucnredlist.org
  59. Jaskani, M. J., Abbas, H., Sultana, R., Khan, M. M., Qasim, M., & Khan. I. A. (2008). Effect of growth hormones on micropropagation of Vitis vinifera L. CV. perlette. Pakistan Journal of Botany, 40(1), 105-109.
  60. http://142.54.178.187:9060/xmlui/handle/123456789/16978
  61. Jones, B., Gunnerås, S.A., Petersson, S.V., Tarkowski, P., Graham, N., May, S., Dolezal, K., Sanberg, G. Ljung, K. (2010). Cytokinin regulation of auxin synthesis in Arabidopsis involves a homeostatic feedback loop regulated via auxin and cytokinin signal transduction. The Plant Cell, 22(9), 2956-2969.
  62. https://doi.org/10.1105%2Ftpc.110.074856
  63. Kareem, A., Radhakrishnan, D., Sondhi, Y., Aiyaz, M., Roy M. V., Sugimoto, K., & Prasad, K. (2016). De novo assembly of plant body plan: a step ahead of Deadpool. Regeneration, 3(4), 182-197. https://doi.org/10.1002/reg2.68
  64. Kothari, S. L., Joshi, A., Kachhwaha, S., & Ochoa-Alejo, N. (2010). Chilli, peppers-A review on tissue culture and transgenesis. Biotechnology Advances, 28(1), 35-48.
  65. https://doi.org/10.1016/j.biotechadv.2009.08.005.
  66. Kumar, K. R., Singh, K. P., Jain, P. K., Raju, D. V. S., Kumar, P., Bhatia, R., & Panwar, S. (2018). Influence of growth regulators on callus induction and plant regeneration from anthers of Tagetes spp. Indian Journal of Agricultural Sciences, 88(6), 970-977.
  67. Kumlay, A. M., & Ercisli, S. (2015). Callus induction, shoot proliferation and root regeneration of potato (Solanum tuberosum L.) stem node and leaf explants under long-day conditions. Biotechnology & Biotechnological Equipment, 29(6), 1075-1084.
  68. https://doi.org/10.1080/13102818.2015.1077685
  69. Lavanya, A. R., Muthukumar, M., Muthukrishnan, S., Kumaresan, V., Kumar, T. S., Rao, A. S., & Rao, M. V. (2016). In vitro micropropagation of Simarouba glauca DC. Indian Journal of Biotechnology, 15(1), 107-111.
  70. http://nopr.niscpr.res.in/handle/123456789/34524
  71. Lee, S., & Huang, W. (2014). Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation. Plant Growth Regulation, 73(2), 193-204. https://doi.org/10.1007/s10725-013-9880-x
  72. Lima, E. M., Curcio, G. R., Bonnet, A., Uhlmann, A., & Palma, V. H. (2018). Crescimento inicial de espécies arbóreas nativas em solos degradados e com presença de plintita no Bioma Cerrado, Brasília - DF. Nativa, 6, 787-794.
  73. http://dx.doi.org/10.31413/nativa.v6i0.6210
  74. Liu, J., Feng, H., Ma, Y., Zhang, L., Han, H., & Huang, X. (2018). Effects of different plant hormones on callus induction and plant regeneration of miniature roses (Rosa hybrida L.). Horticulture International Journal, 2(4), 201-206.
  75. https://doi.org/10.15406/hij.2018.02.00053
  76. Mihaljević, I., Dugalić, K., Tomaš, V., Viljevac, M., Pranjić, A., Čmelik, Z., Puškar, B., & Jurković, Z. (2013). In vitro sterilization procedures for micropropagation of 'Oblačinska' sour cherry. Journal of Agricultural Sciences Belgrade, 58(2), 117-126.
  77. https://doi.org/10.2298/JAS1302117M
  78. Mc-Caughey-Espinoza, D., Ayala-Astorgo, G., García-Baldenegro, C., Buitimea-Cantúa, N., Buitimea-Cantúa, G., & Ochoa-Meza, A. (2020). In vitro germination and induction of callus and root in Bursera laxiflora S. Watson. Abanico Agroforestal, 2, 1-14.
  79. http://dx.doi.org/10.37114/abaagrof/2020.4
  80. Mc-Caughey-Espinoza, D., Reyes-Olivas, Á., Ayala-Astorga, G., Lugo-García, G., Ochoa-Meza, A., & Pacheco-Olvera, A. (2020). In vitro induction of callogenesis and organogenesis in explants of Krameria erecta Willd. Abanico Agroforestal, 2(1), 1-13.
  81. http://dx.doi.org/10.37114/abaagrof/2020.5
  82. Mehbub, H., Akter, A., Akter, M. A., Mandal, M. S. H., Hoque, M. A., Tuleja, M., & Mehraj, H. (2022). Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application. Plants, 11(23), 3208.
  83. https://doi.org/10.3390/plants11233208
  84. Mohd Din, A. R. J., Ahmad, F. I., Wagiran, A., Samad, A. A., Rahmat, Z., & Sarmidi, M. R. (2016). Improvement of efficient in vitro regeneration potential of mature callus induced from Malaysian upland rice seed (Oryza sativa cv. Panderas). Saudi Journal of Biological Sciences, 23(1), S69-S77. https://doi.org/10.1016/j.sjbs.2015.10.022
  85. Moraes, R. M., Cerdeira, A. L., & Lourenço. M. V. (2021). Using micropropagation to develop dedicinal plants into crops. Molecules, 26(6), 1752.
  86. https://doi.org/10.3390/molecules26061752
  87. Murashige, T. & Skoog, F. (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Plant, 15(3), 473-497.
  88. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  89. Naik, N., Rout, P., Umakanta, N., Verma, R. L., Katara, J. L., Sahoo, K. K., Singh, O. N., & Samantaray, S. (2017). Development of doubled haploids from an elite indica rice hybrid (BS6444G) using anther culture. Plant Cell, Tissue and Organ Culture, 128, 679-689.
  90. https://doi.org/10.1007/s11240-016-1149-4
  91. Payghamzadeh, K. Kazemitabar, S. K. (2011). In vitro Propagation of Walnut – A review. African Journal of Biotechnology, 10(3), 290-311. https://doi.org/10.5897/AJB10.324
  92. Pompeu, G. B., Gratão, P, L., Vitorello, V. A., & Azevedo, R. A. (2008). Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture. Sciencia Agricola, 65(5), 548-552. https://doi.org/10.1590/S0103-90162008000500015
  93. Purohit, S.D., Da Silva, T.J., & Habibi, N. (2011). Current approaches for cheaper and better micropropagation technologies. International Journal of Plant Developmental Biology, 5(1), 1-36.
  94. Revilla-Chávez, J. M., Abanto-Rodríguez, C., Guerra, A. W. F., García, S. D., Guerra, A. H., Domínguez, T. G., & Carmo, I. L. G. (2021). Modelos alométricos para estimar el volumen de madera de Guazuma crinita en plantaciones forestales. Scientia Agropecuaria, 12(1), 25-31. http://dx.doi.org/10.17268/sci.agropecu.2021.003
  95. Reynel, C., Pennington, T. D., Pennington, R. T., Flores, C., & Daza, A. (2004). Miradas botánicas y miradas forestales a los árboles y madera en la Amazonía peruana. Revista Peruana de Biología, 11(1), 113-114. https://doi.org/10.15381/rpb.v11i1.2443
  96. Roberts, S., & Kolewe, M. (2010). Plant natural products from cultured multipotent cells. Nature Biotechnology, 28, 1175-1176. https://doi.org/10.1038/nbt1110-1175
  97. Rodriguez, H., Geistlinger, J., Berlyn, G., Kahl, G., & Weising, K. (2000). Characterization of novel microsatellite loci isolated from the tropical dioecious tree Simarouba amara. Molecular Ecology, 9(4), 498-500.
  98. https://doi.org/10.1046/j.1365-294x.2000.00871-7.x
  99. Rodríguez, M. M., Latsague, M. I., Chacón, M. A., & Astorga, P. K. (2014). In vitro induction of callogenesis and indirect organogenesis from explants of cotyledon, hypocotyl and leaf in Ugni molinae. Bosque (Valdivia), 35(1), 111-118.
  100. http://dx.doi.org/10.4067/S0717-92002014000100011
  101. Ronquillo, M.G.L. (2005). Inducción de callogénesis in vitro a partir de láminas foliares de Sansevieria trifasciata. Thesis, Ing. Agronomo, Escuela Agrícola Panamericana, Carrera de Ciencia y Producción Agropecuaria. 30 pp.
  102. Salem, J., Hassanein, A., El-Wakil, D. A., & Loutfy, N. (2022). Interaction between Growth Regulators Controls In Vitro Shoot Multiplication in Paulownia and Selection of NaCl-Tolerant Variants. Plants, 11(4), 498. https://doi.org/10.3390/plants11040498
  103. Santos, J. P. A., De Souza, M. O., Souza, J. S., Da Silva, R. R., & Mendonça, A. V. R. (2022). Production time and container size for Simarouba amara Aubl. seedlings. Floresta e Ambiente, 29(3), e20220030.
  104. https://doi.org/10.1590/2179-8087-FLORAM-2022-0030
  105. Sarropoulou, V., Sperdouli, I., Adamakis, I. D., & Grigoriadou, K. (2022). The use of different LEDs wavelength and light intensities on in vitro proliferation of cherry rootstock: influence on photosynthetic pigments, photosystem II photochemistry and leaf anatomy. Plant Cell Tissue Organ Culture, 1, 1-22.
  106. https://doi.org/10.21203/rs.3.rs-1789324/v1
  107. Shen, H. J., Chen, J. T., Chung, H. H., & Chang, W. C. (2018). Plant regeneration via direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’. Botanical Studies, 59(4), 1-8. https://doi.org/10.1186/s40529-018-0220-3
  108. Shekhawat, M. S., & Manokari, M. (2016). Impact of Auxins on Vegetative Propagation through Stem Cuttings of Couroupita guianensis Aubl.: A Conservation Approach. Scientifica (Cairo), 2016, 1-7. https://doi.org/10.1155/2016/6587571
  109. Da Silva, J. A. T., Kulus, D., Zhang, X., Zeng, S., Ma, G., & Piqueras, A. (2016). Disinfection of explants for saffron (Crocus sativus) tissue culture. Environmental and Experimental Biology, 14(4), 183-198. http://doi.org/10.22364/eeb.14.25
  110. Silva, T. S., Nepomuceno, C. F., Borges, B. P. S., Alvim, B. F. M., & Santana, J. R. F. (2013). In vitro multiplication of Caesalpinia pyramidalis (Leguminosae). Sitientibus série Ciências Biológicas, 13, 2-6. https://doi.org/10.13102/scb320
  111. Soudre, M., Vidal, F., Mori, J., Guerra, H., Mesen, F. Perez. F. (2010). Vegetative propagation of marupa (simarouba amara aubl.) by rooting of juvenile cuttings in a non-mist propagator, Folia Amazónica, 19(1-2), 61-68. https://doi.org/10.24841/fa.v19i1-2
  112. Souza, M. M., Bufalino, L., & Gomes, L. G. (2020). Wood characterization of Marupá (Simarouba Amara Aubl, Simaroubaceae) for use in the furniture industry. Brazilian Journal of Development, 6(12), 98163-98185. https://doi.org/10.34117/bjdv6n12-347
  113. Su, Y. H., Tang, L. P., Zhao, X. Y., & Zhang, X. S. (2021). Plant cell totipotency: Insights into cellular reprogramming. Journal Integrative Plant Biology, 63(1), 228-243.
  114. https://doi.org/10.1111/jipb.12972
  115. Tabiyeh, D. T., Bernard, F., & Shacker, H. (2006). Investigation of Glutathione, Salicylic acid and GA3 Effects on Browning in Pistacia vera Shoot Tips Culture. Acta Horticulturae, 726, 201-204. https://doi.org/10.17660/ActaHortic.2006.726.31
  116. Tang, W., & Newton, R. (2004). Increase of polyphenol oxidase and decrease of polyamines correlate with tissue browning in Virginia pine (Pinus virginiana Mill.). Plant Science, 167(3), 621-628. https://doi.org/10.1016/j.plantsci.2004.05.024
  117. Van Staden, J., Fennell, C. W., & Taylor, N. J. (2006). Plant Stress In Vitro: the Role of Phytohormones. Acta Horticulturae, 725, 55-62.
  118. https://doi.org/10.17660/ActaHortic.2006.725.2
  119. Vatanpour-Azghandi, A., Villiers, T. A., Ghorbani, A. M., & Tajabadi, A. (2002). The microscopy of tissue decolouration and browning problem in pistachio callus cultures. Acta Horticulturae, 591, 377-388. https://doi.org/10.17660/ActaHortic.2002.591.58
  120. Wang, Y. H., & Bhalla, P.L. (2004). Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br. Plant Cell Reports, 22(6), 408-414.
  121. https://doi.org/10.1007/s00299-003-0707-5
  122. Wu, W., Du, K., Kang, X., & Wei, H. (2021). The diverse roles of cytokinins in regulating leaf development. Horticulture Research, 8(1), 118.
  123. https://doi.org/10.1038/s41438-021-00558-3
  124. Qin, T., Shu, X., Zhuang, W., Peng, F., & Wang, Z. (2017). High Efficiency Callus Induction and Regeneration of Solanum torvum Plants. HortScience, 52(12), 1755-1758.
  125. https://doi.org/10.21273/HORTSCI12232-17
  126. Zanoni-Mendiburu, C. A. (1975). Propagation of filberts by stem cutting. Comb. Proc. Intnatl. Plant Propagators´ Soc. 41, 214-218.
Efecto de la desinfección, el desarrollo foliar y las fitohormonas en la inducción de callos de Simarouba amara Aubl. (2024). Ciencia Y Tecnología Agropecuaria, 25(3). https://doi.org/10.21930/rcta.vol25_num3_art:3662
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